Coding

Part:BBa_K2220021:Design

Designed by: Nan Rong   Group: iGEM17_BNU-China   (2017-10-22)


Secrete mCherry protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 286
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 244
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1066
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1048


Design Notes

We have done the codon optimization.


Source

Alpha-tubuin http://www.ncbi.nlm.nih.gov/nuccore/393715088 Aga2 comes from pYD1 plasmid.


References